Researchers from the Centre of the Region Hana for Biotechnological and Agricultural Research ( CRH ) in Olomouc , together with colleague from the University of Nottingham , and the Max Planck Institute of Molecular Cell Biology in Dresden , have made another significant step in exploring plant structure and exploitation using light sheet fluorescence microscopy . From the traditional model plant - Arabidopsis thaliana , they upgrade to a much larger Medicago sativa , which can be observed in its rude state for up to several days . unparalleled experiences have been sum up in an article for the prestigious diary Nature Plants , which adumbrate also the perspectives of the method to the reality . In the futurity , they stand for to employ it for other craw , specially barleycorn . get information is crucial , for example , for increasing plant return or resistance to adverse environmental condition , let in drouth or disease .
The stepping stone of this inquiry were elaborated protocol for foresightful - term and gentle exploration of plant with high - resolution using commercially available optical maser microscopes , which CRH scientist put out three years ago in Nature Protocols , attract considerable sake in the scientific biotic community . Subsequently , researchers have decided to break and modify the technique for long - term and gentle geographic expedition of industrial plant so that they can capture objects larger than Arabidopsis , such as alfalfa embryos and plant life develop from them .
" We have follow in further improvement of the only method that can be used to visualize life plants at different levels from intracellular to the whole plant . The fact that we have set ahead to alfalfa from Arabidopsis is a great milepost . We can follow the plant development from several hours to several days . We can observe not only its development but also its interactions with international surround and microbes in defined and strictly controlled circumstance . We will get much better explanations to a range of biologic and biotechnological questions , " enounce Jozef Samaj , head of Olomouc research mathematical group .
According to his workfellow and a co - author Miroslav Ovecka , the transfer of the method from the model flora to alfalfa was technically quite complex . " We apply a commercially usable microscopical system , so we had to take into account its expert parameter . It was important to cook conditions for long - term plant cultivation . The plant life in the microscope receive fresh nutrient in a uninspired environment , is supplied with the air and exposed to the normal light / dark illumination cycle . The results are not affect by secondary strain triggered by microscopy itself . The significant shift in the scientific field of honor lend about by this method acting lies in the fact that there has not yet been a system approach shot that would evaluate alfalfa at such a level . We have also described technological difficulty that other research groups often face , and suggested a way how this progressive method acting could be used for developmental discipline for any type of works objects . Also from this point of view , the article is singular . We are present new perspectives to the scientific residential area based on receive effect , " explained Ovecka .
Olomouc scientist intend to keep to improve the methodology and conform it for possible use in barleycorn within project " plant as a means of sustainable global evolution " under Operational Program for Research , Development and Education . Thanks to a theme in a esteemed diary , they will be able to present the progress in this proficiency and its possible applications in plant life enquiry at even more conferences and professional seminar . CRH bring together scientific team from Palacky University and Olomouc worksites of the Institute of Experimental Botany of Academy of Sciences of Czech Republic , and the Crop Research Institute .
For more information : https://www.nature.com / articles / s41477 - 018 - 0238 - 2
